FAQ: After use, how can I inactivate NEBExpress^® Salt Active Nuclease?

NEBExpress Salt Active Nuclease can be inactivated by one of the following methods:

1. Heat inactivation: Incubate at 65C for 2 h, at 80C for 1 h, or at 95C for 20 min.
   
   
2. Chemical inactivation: Add > 10 mM EDTA, or 0.5% SDS, or > 20 mM TCEP.
   
   
3. Chemical + heat inactivation: Add 1 mM DTT and incubate at 55C for 30 min.
   
   
4. Enzymatic degradation: Add Proteinase K (NEB #P8107S) or Thermolabile Proteinase K (NEB #P8111S).