FAQ: What library sizes can be generated with the TnX® Transposase (Unloaded) (NEB #M2623)?

Library size is influenced by DNA input amount, quality, transposome concentration, and reaction conditions e.g. choice of enzymes/workflows. Under standard unit assay conditions, loaded TnX transposome generates average library sizes of approximately 350-450 bp. Users can adjust reaction parameters to tailor fragment size distributions for specific downstream applications.