mRNA-Seq or Total RNA-Seq: How Sample and Question Shape the Choice - Biocompare

RNA next-generation sequencing is a helpful tool for scientists to analyze samples — but how do researchers decide what NGS approach to choose? Biocompare Custom Content Director Niki Spahich, Ph.D., breaks down what to consider when choosing between mRNA-seq or total RNA-seq approaches. NEBNext Product Marketing Manager Deyra Rodríguez, Ph.D., contributed comments on the decision.

Different research questions lend themselves to different NGS approaches

Rodríguez highlights that mRNA-seq and total RNA-seq profile different RNA populations, so the sequencing approach depends on the question a scientist is asking. When asking questions that involve multiple RNA classes, combining library preparation approaches can be helpful.

Samples favor different NGS methods

In addition to the specific research question, Rodríguez points out that different samples work best with different approaches.

Choosing between breadth and depth

While total RNA-seq provides information on more kinds of RNA, some research questions require increased sequence depth. Balancing the range of RNA populations needed and the sequencing detail is crucial to NGS workflows.

In the full article, read more about considerations when choosing what NGS approach is best for a project and more.

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