Methyl-DNA immunoprecipitation (Me-DIP) and Methyl-CpG Binding Domain (MBD) -based capture are both effective methods to enrich methylated DNA from genomic DNA samples. However, for genome-wide methylation analysis, these two methods have differing CpG density bias. Me-DIP favors low methyl CpG density whereas MBD favors higher methyl CpG density (1). Both methods can be used upstream of microarray or sequencing analysis.
- Shalima S. Nair et al. (2011) Epigenetics 6:1,34-44. PMID: 20818161