Extraction-Free PCR
The presence of intrinsic PCR inhibitors in some sample types, such as those from blood or fixed tissues, can prevent the direct amplification of DNA from these samples. DNA can be purified prior to PCR via a variety of extraction techniques but these methods can be costly, time intensive and result in significant sample loss. Extraction-free PCR typically benefits from a polymerase that is resistant to inhibitors, allowing for robust amplification of source DNA without additional extraction steps.
FAQs for Extraction-Free PCR
Protocols for Extraction-Free PCR
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Anatomy of a Polymerase - How Structure Effects Function
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Polymerase Fidelity: What is it, and what does it mean for your PCR?
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Understanding Variability in DNA Amplification Reactions
- DNA Polymerase Selection Chart
- PCR Troubleshooting Guide
- Taq PCR Kit Troubleshooting Guide
- General Guidelines for Successful RNA Purification Using the Monarch Total RNA Miniprep Kit
- Guidance on Choosing Sample Input Amounts when Using the Monarch Total RNA Miniprep Kit
- Guidelines for PCR Optimization with OneTaq® and OneTaq® Hot Start DNA Polymerases
- Guidelines for PCR Optimization with Taq DNA Polymerase
- Guidelines for PCR Optimization with Thermophilic DNA Polymerases
Other Tools & Resources
Feature Articles
Selection Tools
Troubleshooting Guides
Usage Guidelines
Legal Information
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
