Helicase-dependent Amplification

Helicase-dependent amplification (HDA) employs the double-stranded DNA unwinding activity of a helicase to separate strands, enabling primer annealing and extension by a strand-displacing DNA polymerase. This method was originally developed at NEB and is now used in a number of FDA-approved diagnostic assays. Like PCR, this system requires only two primers and produces short, discrete DNA products.

Reaction Temperature Amplicon Size Detection Method(s)
 65°C  <150 nt  Lateral flow

Helicase-Dependent Amplification Workflow

HDA utilizes a helicase to separate DNA in the presence of single-stranded binding proteins, enabling primer annealing and extension by a strand-displacing DNA polymerase.

 


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