Video Library

In this episode of NEB TV, learn about some exciting projects that help to promote STEM education. more

In Episode 3, learn more about amplification, including new techniques to bring amplification into the field. Also, learn more about high-fidelity DNA polymerases, and how we responded to a recent tech support question on the subject. more

On this episode of NEB TV, we’re talking about synthetic biology. What is synthetic biology, who’s using synthetic biology, and where can synthetic biology take us in the not-too-distant future? more

Whether you are new to library preparation for NGS, using reagents from another supplier, or sending samples to a core facility, it is important to know what it takes to get high-quality libraries. Learn more about library preparation and hear where next generation sequencing is going in the latest episode of NEB TV. more

Learn how NEB’s Customized Solutions Team combines technical expertise, commitment to quality and scale of manufacturing to provide a unique solution for your product or technology. more

In this video, find a summary of the publication from NEB scientists describing the application of loop-mediated isothermal amplification to a field test to detect Mansonella infection. more

Learn how NEB helped a growing life science company with product formats and sourcing, ultimately decreasing workflow time and cost. more

Learn how NEB helped a startup company manufacturing a novel therapeutic with GMP-grade* enzyme. more

Find out now NEB was able to customize a master mix used in RT-LAMP for the CDC, enabling Zika-based testing. more

Learn how NEB can streamline your supply chain through our Vendor Managed Inventory (VMI) program. more

In this episode, we discuss the importance of biodiversity and conservation efforts aimed at its protection, including how we can all take part in this effort. Also, learn about Ocean Genome Legacy Center (OGL) and their dedication to exploring and preserving biodiversity in the ocean. more

Looking to gain some background in molecular biology? Hear why our Molecular Biology Summer Workshop might be just what you need. more

What does Passion in Science mean to you? Learn how NEB celebrated 15 scientists' passion projects with the 2014 Passion in Science Awards. more

Did you know that NEB offers free reagents and support to teaching labs and educational courses in the US? In this episode, learn more about NEB’s course support program, hear from various organizations who have taken advantage of this benefit, and learn how educators can participate. more

See Dr. Eva Nogales present her work on the "Structure and Dynamics of Macromolecular Complexes using Cryo-EM." more

Join us for the full 2019 Passion in Science Awards® Ceremony and enjoy a presentation from each of the award categories: Arts and Creativity, Science Mentorship and Advocacy, Humanitarian Duty and Environmental Stewardship. more

Learn how to submit a request for reagents to support your high school or collegiate teaching labs and educational courses in the US. View the request forms at: https://www.neb.com/promoting-science-education/course-support-and-reagent-donation more

Learn how the myNEB Skill for Alexa can help life science researchers at the bench with restriction enzyme information, scientific calculations and more. more

Take an aerial tour of the New England Biolabs campus and see inside both the historic and laboratory buildings. more

In this episode, we discuss the challenges associated with current methods available for methylome analysis, and introduce NEBNext Enzymatic Methyl-seq (EM-seq™) as an alternative to bisulfite sequencing that addresses these challenges. more

Learn more about the Genes in Space initiative and hear about the 2015 winner’s project, which utilized DNA polymerases from NEB to study epigenetic changes during spaceflight. more

What is Passion in Science? Hear what some NEB employees have to say on the topic, and learn more about our Passion in Science Awards. more

Have you ever stopped to think about how much potential recycling a typical researcher creates over the course of a day? Learn more in this video. more

Learn how to purchase products from an NEBnow freezer. It’s as quick and easy as 1, 2, 3… more

Learn how to restock your NEBnow freezer. It’s as quick and easy as 1, 2, 3… more

As a scientist, there is nothing worse than running out of your favorite enzyme in the middle of an experiment. NEBnow’s self-service kiosk enables our products and your discoveries to always be within reach. more

Managing a stockroom or core facility is a lot of work. NEBnow makes it easier by automating inventory management and logistics, all while providing outstanding service. more

Hear more about the inspiring projects of the 2014 Humanitarian Duty Award winners more

2014 New England Biolabs Environmental Stewardship Award winners define "Passion in Science"

Learn more about the intersection between art and science from our 2014 Arts and Creativity Award winners more

Hear from our 2014 Inspiration in Science Award winners about what passion in science means to them. more

Whether you are starting your job search in the life sciences, looking for a background in molecular biology, or exploring alternative career paths, this episode is for you. more

Traditional cloning or DNA assembly? Learn more about the benefits of each in the latest episode of NEB TV. more

Science in 60 – Molecular Biology Summer Workshop more

Can laboratories become more “green"? In this episode of NEB TV, find tips on how you can help to reduce your environmental footprint in the lab, and hear about some NEB initiatives, including a unique approach for wastewater treatment and the development of our new environmentally friendly DNA purification kits. more

Hear more about what the iGEM competition is all about from an NEB scientist. more

Get helpful tips and tricks for job hunting, interviewing and resume building from NEB scientists more

Passion in Science Environmental Stewardship Award winner, Samantha Romanick, describes her journey to a low waste lifestyle, and her work to reduce plastic usage by founding the Campus Refill Initiative, which offers alternatives to single-use plastics. more

Passion in Science Mentorship and Advocacy Award winner, Steven Farber, describes his not-for-profit organization called BioEYES that uses Zebrafish to educate K-12 students about science. more

Passion in Science Humanitarian Award winner, Nathan Schoepp, describes his research to develop rapid point-of-care diagnostics to determine antibiotic resistance in clinical samples. more

Precision medicine requires precision enrichment. Learn more about target enrichment and its role in clinical applications. more

Passion in Science Arts and Creativity Award winner, William Murta, is the composer of Das Molekul – a musical about the discovery of DNA and the race to sequence the human genome. more

Interested in learning more about how NEB’s enzymes are made? Learn more in this episode of NEB TV. more

DNA assembly is growing in popularity and used in a number of applications. Find out how NEB customers are using DNA assembly and our NEBuilder HiFi products in this episode of NEB TV. more

Does creativity inspire you to be a better scientist? Learn more about the often unpredictable ways that art and science intersect in the latest episode of NEB TV. more

Alexandra Polasko, graduate student at UCLA, shares her passion for providing clean drinking water through the process of bioremediation. Alexandra explains how bioremediation uses microbes to degrade contaminants and describes her development of lost cost, rapid indicator technology to identify naturally occurring bacteria capable of degrading hazardous pollutants. more

Scott Chimileski, research fellow at Harvard Medical School, shares his passion for communicating the value of microbes through photography. Scott discusses the book he is currently co-authoring with Roberto Kolter and he describes the exhibit he is designing for Harvard Museum of Natural History, both of which aim to communicate the beauty, history and power of microbes through engaging photography. more

Saban Ul-Hasan, graduate student at University of California Merced, shares her passion for inspiring understanding of symbiotic relationships between scientists, artists, the public and the environment. Sabah promotes these relationships through production of the educational video series “BIOTA”, which highlights specific ecosystems and features music of local bands and work of local artists. more

Lucy Lahrita, of Hokkaido University, Japan, shares her passion for protecting the environment by utilizing chemical recycling to create synthetic fuel from plastic waste. Lucy describes how specific types of plastic can be used to not only create crude oil, but a new job sector as well. She encourages us all to think globally and act locally to protect our environment. more

David Ng, Director of the Science Literacy program at Michael Smith Labs of the University of British Columbia, shares his passion for public understanding of science. David explains how he promotes science communication through workshops, educational tools and research studies, and suggests that all scientists find a genuine way to advocate science and engage the public. more

Professor Chris Martine shares his passion for inspiring exploration and discovery in botany and the natural world. Chris connects with students at Bucknell University and beyond with humor and science-rich content through his video series “Plants are Cool, Too!”. more

Professor Amanda Munson of Shenandoah University shares her passion for inspiring girls to pursue careers in STEM fields. Amanda describes GO GIRL™ (Genomic Opportunities for Girls in Research Labs), an education outreach program designed to boost confidence and provide hands-on science experience to high school girls in a research laboratory with state-of-the-art equipment. more

Scott O’Neill shares his passion for developing novel approaches to controlling mosquito transmitted viruses by introducing Wolbachia infections in mosquitos. Scott describes the troubling rise in mosquito-borne viruses, and offers the promising results of his work with Eliminate Dengue. more

Nina Dudnik shares her passion for providing global access to scientific research equipment and collaboration. Nina founded Seeding Labs to support scientific discovery and education in the developing world through financial resources, research equipment and access to expertise. She explains how providing repurposed equipment has allowed labs to educate the next generation of scientists, secure funding to support future research, and empower scientists in developing countries. more

Lucia Prieto Godino shares her passion for providing access to science education and hands-on training in Africa. Lucia founded TReND in Africa to support global science efforts through educational course training, equipment donations and encouragement of international collaboration. more

Shelly Xie shares her passion for science, art and humanity. Shelly illuminates personal stories of global health issues through sand animation. Enjoy this premiere of Shelly’s work created in collaboration with Pan American Health Organization (PAHO). more

Lisa Anderson, post-doc at MIT, shares her passion for making the world a better place through science and technology. Lisa discusses the laboratory glove recycling program that she helped to create while at University of California Davis. She also describes green lab assessment programs and her current efforts at MIT. more

Dana Simmons, graduate student at The University of Chicago, shares her passion for sharing the beauty of science and the complexity of the human neural system through photography. Dana explores the similarities between nature and biology by introducing fluorescent dye into purkinje cells and capturing striking images after the completion of her scientific experiments with the cells. Dana elevates her science to art form and reaches a broader audience with her efforts. more

Christine Liu, graduate student at University of California Berkeley, shares her passion for communicating complex science topics to a broad audience through zines. Christine co-founded Two Photon Art collective, and she describes how the informal presentation style of zines makes science more approachable and less intimidating. more

Karishma Bhagani, undergraduate student at University of New York, shares her passion for providing access to clean drinking water in countries suffering from water scarcity. Karishma created the aspiring non-profit organization Matone de Chiwit, which provides economical water purification units comprised of domestically available elements to rural communities. more

Will Ryan, graduate student at Florida State University in Tallahassee, shares his passion for communicating seminal scientific literature in engaging short films. Will distills the take-home messages of the literature and delivers these messages through poetry and art in his films, making science literature less intimidating and more relatable for his audience. more

Take a trip with us to the Harvard Museum of Natural History for a behind the scenes tour of Scott Chimileski and Roberto Kolter’s photographic journey into the microbial world. more

In this episode of NEB TV, find tips for greening your lab, hear from the 2017 iGEM Goes Green team, and learn more about Labconscious, a site devoted to sustainability in the lab. more

NEB’s Chief Scientific Officer, Sir Richard Roberts, describes the Enzymes for Innovation initiative, and the importance of new enzyme discovery and screening. more

These days, restriction enzymes are being used in many more applications other than cloning. Learn more in this episode of NEB TV. more

2017 Genes in Space Winner Sophia Chen designed an experiment to study the effects of DNA damage in astronauts and their susceptibility to cancer. more

2017 Genes in Space Winner Liza Reizis designed an experiment to study the immune system of astronauts to better understand why they are more likely to get sick. more

In this video, learn how to teach your elementary aged kids about density, volume and mass. more

In this video, learn how to teach your elementary aged kids to extract DNA from strawberries. more

Several of the most important neglected tropical diseases in humans are the result of infection with filarial nematode parasites. In this episode of NEB TV, learn more about these diseases and their impact, and how understanding their biology can help identify methods to detect, control and treat these parasites in the field. more

In this video, learn how to teach your elementary aged kids about the diversity of organisms found in pond water. more

In this video, learn how to teach your elementary aged kids how to solve a crime with chromatography and microscopy. more

In this video, learn how to teach your elementary aged kids about surface tension. more

In this video, learn how to teach your elementary aged kids about some properties of acids and bases. more

In this video, learn how to teach your elementary aged kids about catalysts. more

In this video, learn how to teach your elementary aged kids about what yeast likes to eat to make bread rise. more

In this video, learn how to teach your elementary aged kids about the molecular structure of water. more

In this video, Tilde Carlow describes the aims and interests of the Genome Biology Research Division at NEB. more

In this video, Andy Gardner describes the aims and interests of the Molecular Enzymology Research Division at NEB. more

In this video, Jennifer Ong describes the aims and interests of the Nucleic Acid Research Division at NEB. more

In this video, Chris Taron describes the aims and interests of the Protein Expression & Modification Research Division at NEB. more

In this video, Brett Robb describes the aims and interests of the RNA Research Division at NEB. more

Learn more about the benefits of our New Student Starter Pack Program from our NSSP Specialist, Kari Goodwin. more

Learn how NEB's ISO-certified manufacturing facility is equipped to handle the demands of your bulk, custom, or OEM product order. more

Are you worried that your question is too basic to call NEB technical support? Nathan offers some reassurance. more

Can you guess how many tech support inquiries NEB receives annually? We think you’ll be surprised! more

What’s the best way to get in touch with NEB’s tech support? Whether you call or email, you’ll get answers from bench scientists who have experience with the product! more

A scientist-run technical support program is the hidden value behind NEB’s reagents. Learn how you can benefit! more

Nowhere does NEB’s “scientists helping scientists” philosophy shine more than in conversation with two NEB scientists who regularly answer customer questions. more

Jeremy’s “most interesting call” story highlights the full panoply of resources available when you call with a tech support question. more

The questions posed to NEB tech support run the gamut from basic procedural and historical questions to complex issues about cutting-edge applications. Our scientists are ready and willing to help! more

Are you interested in either in-licensing or out-licensing opportunities for biotechnology tools? Learn more about NEB’s Global Business Development department. more

Learn how New England Biolabs sets a new standard for life science reagents. more

Go behind the scenes into NEB’s technical support program. Hear from our scientists as they describe memorable tech support moments and interesting collaborations that have resulted from tech support calls. more

In this video, learn more about argonautes, including how these nucleic acid-guided endonucleases differ from Cas enzymes and potential applications. more

In this episode, learn about new and interesting techniques in science communication from our 2019 Passion in Science Winners. more

In this episode, experts share their insights on best practices for sustainable laboratories with scientists

Learn more about our new 100% recyclable solution for cold chain shipping of your NEB products. more

Meet New England Biolabs more

Learn how NEB protects the environment, both locally and globally. more

Learn how our state-of-the-art Solar Aquatics System® treats the NEB campus’ wastewater. more

Cell-free protein expression enables the synthesis of a wide variety of proteins in just a few hours, and can be used in a variety of applications, including protein engineering, expression of toxic proteins and diagnostic assays. more

One little proton could change the way scientists detect DNA amplification in the field and point-of-care settings. Nathan shares the details of his recent paper. more

Did you know that this isothermal amplification method can be performed in as little as 5-10 minutes with limited resources? Get a quick overview on how LAMP works in this animation. more

Making parasite diagnostics more accessible to clinicians in the field could help to eradicate neglected tropical diseases. Andy describes the findings of his recent paper. more

Learn the basics of qPCR in this short animation. more

Learn how we analyzed plates and plates of qPCR data, and how Luna stacks up to the competition. more

Isothermal amplification generates many copies of a target sequence in a short period of time, at a constant temperature. Learn more about isothermal amplification. more

Jennifer Ong explains her recent NEB publication demonstrating how single-molecule PacBio sequencing was used to better understand sources of error introduced by PCR. more

Hear the 2016 Genes In Space winner describe his project and how Colorimetric LAMP will be used in space to examine accelerated aging. more

Learn the basics of Colorimetric LAMP, and how groups like NEB and the World Mosquito Program are using this isothermal amplification method in field tests and point-of-care diagnostics. more

This video gives tips on how to determine the best annealing temperature for your primers using the NEBaseChanger tool. more

This video explains what to do if you find a precipitate in your master mix. more

This overview will walk you through how the Polymerase Chain Reaction (PCR) works. more

Experiencing amplification frustration? Follow Melanie’s 5 quick and easy tips for PCR setup to improve your yields. more

Learn how proofreading polymerases recognize and correct mismatched bases. more

For the inside scoop on how NEB products come to be, learn the story behind the new NEB® PCR Cloning Kit. more

Make sure you're using the optimal polymerase for your DNA amplifications. Get tips on choosing the right DNA Polymerase for your application. more

Looking for tips on dealing with GC-bias in DNA amplification? NEB scientists have the expertise you need! more

View the narrated animation more

Why do primer melting temperatures matter? How is an annealing temperature calculated? Nicole has answers to these questions & more, in this quick video! more

Here are some quick tips for getting the most out of NEB's Q5® High-Fidelity DNA Polymerase. more

Not sure why Q5® is your best choice for high-fidelity amplification of GC-rich targets? NEB's scientists will show you why we call Q5 an "ultra-high fidelity polymerase". more

The secondary structures that form within GC-rich regions can make efficient DNA amplification for PCR difficult. Nicole’s suggestions can help! more

Do you need to optimize your PCR of large amplicons? Becky shares some tips for choosing the right polymerase and cycler conditions, as well as optimal handing methods, to get the most out of those long templates!

High-fidelity, multiplex, and Fast PCR are just a few of the types of amplification reactions you may need to conduct. NEB offers a variety of DNA polymerases for all of your experimental needs. Breton explains which polymerases to choose. more

Traditional Cloning refers to the generation of DNA fragments using restriction enzymes, and their subsequent assembly into vectors and transformation. The name is derived from the method’s history as the first widely-accepted cloning method. Learn more in this tutorial about the benefits and disadvantages of Traditional Cloning. more

This video walks you through DNA Library Preparation using the NEBNext Ultra II DNA Library Prep Kit. The video also includes tips for optimization as well as safe stopping points. more

Learn how Biomek automation of NEBNext DNA library preparation for Ion Torrent enables high-throughput, reproducible production of libraries with high yield and complexity. In this webinar Joanna Hamilton from Dartmouth Genomics & Molecular Biology Shared Resource, Lynne Apone from New England Biolabs and Zach Smith from Beckman Coulter describe the NEBNext Fast Fragmentation & DNA Library Prep kit for Ion Torrent, automation of this method and implementation in multiple workflows in a sequencing core lab. more

Learn how Biomek automation of NEBNext RNA library preparation enables high-throughput, reproducible production of libraries with high yield and complexity. In this webinar Zach Smith from Beckman Coulter describes strand-specific RNA library preparation using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina and discusses Biomek automation of this method. more

Can microbiome-enrichment techniques be used to partition plant DNA? Erbay describes the findings of his recent paper. more

Here are some important things to keep in mind when preparing the NEBNext® Ultra II FS DNA reaction buffer and enzyme mix for use. more

Learn how to prevent media carryover in cell sorting if you are unable to sort directly into the NEBNext Cell Lysis Buffer with murine RNase inhibitor. more

Learn why it is important that the Bead Reconstitution Buffer be at room temperature for use. more

Learn why vortexing the NEBNext Single Cell RT Buffer is so important. more

During the NEBNext Single Cell/Low Input RNA library prep workflow, adding lysis buffer to the cDNA PCR reaction increases efficiency. more

Learn how to make a master mix of the NEBNext Ultra II FS DNA reaction buffer and enzyme mix. more

 Learn how to best preserve RNA in your samples when freezing cells in the NEBNext Single Cell/Low Input RNA library prep workflow. more

The NEBNext^® Magnetic Separation Rack enables efficient bead separation, quickly pulling beads to the side, and allowing elution in small volumes. more

Get a quick overview of NGS library preparation from an NEB scientist. more

View our tutorial for help visualizing NEBNext Direct’s unique and enabling workflow. more

This video walks you through the size selection and cleanup steps using the NEBNext Ultra II DNA more

Learn more about NEBNext Direct and this innovative approach to target enrichment for next-generation sequencing. more

Learn more about the streamlined workflow for the NEBNext Ultra II Directional RNA Library Prep Kit. more

In this webinar, Laurence Etwiller and Jennifer Ong discuss their recent publications, and how library preparation affects sequencing accuracy and variant calling. View full Q & A summary. more

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In this webinar, we explore the advantages and limitations of target enrichment, and discuss how NEBNext Direct^®, a novel solution for selective enrichment of genomic targets, addresses these challenges. 

This video will help you optimize fragmentation for RNA workflows in the NEBNext product line. more

This video gives tips on how to optimize your adapter ligation reaction. more

This video explains the ideal time to elute your SPRI beads. more

This video will help you decide if you need to do a size selection or a simple clean-up for the NEBNext Ultra DNA or RNA. more

This video explains what to do if you find precipitate in your end repair reaction buffer. more

This video will explain why there is sometimes foam when mixing the NEBNext reagents. more

This video provides tips on how to avoid adapter dimer. more

This video describes some things to take into consideration when deciding on a size range for your DNA fragmentation. more

This video explains what PCR overcycling is, how to identify it and how to avoid it. more

This video describes the factors that need to be taken into consideration when calculating your adapter dilution. more

These 12 quick tips for NGS library preparation are a great refresher if you're a seasoned pro, or a great introduction if you're a beginner. more

Using beads to clean up your DNA prior to NGS library prep can be quick and easy, if you follow these few, simple tips. more

Having trouble deciding which NEBNext Fast DNA Library Prep Set for Ion Torrent^® to choose? Let Christine explain which set is right for your DNA. more

The common problem of adaptor dimer formation during small RNA library construction can be avoided by using NEBNext® protocols. Learn about this technique, and how it improves both performance and sensitivity in library construction. more

Not sure how sets and kits differ from modules? Ping explains the difference, and helps you to decide which one is right for you. more

Learn how NEBNext® Ultra is enabling library prep for multiple applications, with lower input amounts and fast, streamlined workflows. In this GenomeWeb webinar Cynthia Hendrickson from HudsonAlpha Institute of Biotechnology, Momo Vuyisich from Los Alamos National Laboratory and Daniela Munafo from New England Biolabs describe use of NEBNext Ultra in exome sequencing, bacterial genome resequencing and assembly, and strand-specific RNA-Seq. more

Are you running a core sequencing facility? Watch as Eileen shares a few reasons that NEBNext products are particularly well suited to sequencing core labs. more

Optimizing DNA inputs for NGS library prep is an important step, if you want to ensure a high-quality library. Start by following these tips for DNA quantification and assessing purity. more

Watch as Deyra explains how to optimize your RNA inputs for successful NGS library preparation. more

A workflow animation for an enzyme-based method for detection of 5mC and 5hmC, an alternative to bisulfite sequencing. more

See how Grozdanov & Clinton, NEB/JoVE video abstract contest winners, have been using NEB's Gibson Assembly products in their laboratory! more

Learn about exonucleases and some of their common applications. more

This webinar reviews the activities of exonucleases and endonucleases, provides insight on how to choose the right exo- or endonuclease for various molecular biology applications, and explains how to use these reagents when developing new molecular biology workflows. more

NEBuilder Assembly Tool can be used to design primers for your NEBuilder HiFi or Gibson Assembly reactions based on entered fragment sequences and the polymerase being used for amplification. This video will highlight some useful updates and the main differences between the two versions. more

This video demonstrates how the NEBuilder Assembly Tool can be used to generate overlap sequences for the assembly of two fragments into a vector. In this scenario, all fragments are amplified by PCR and the vector does not have convenient restriction sites. more

This video demonstrates how to use the NEBuilder® Assembly Tool to build a construct using a restriction enzyme digested vector and two PCR-generated inserts.  The tool will help to design PCR primers containing the required overlap sequences.  We will also regenerate one of the restriction enzyme recognition sites. more

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NEB and NIST scientists devised a single-molecule assay to quantitatively measure long-range DNA unwinding by individual helicase enzymes. Kelly shares details of this recent publication. more

High fidelity polymerases are everywhere—but why would you need a high fidelity ligase? And what do we even mean by “fidelity” when we’re talking about ligation? In this webinar, NEB Scientist and ligase expert Greg Lohman discusses mismatch ligation by DNA ligases and the molecular diagnostics applications that depend on the use of high-fidelity DNA ligases like NEB’s HiFi Taq DNA Ligase to detect single base differences in DNA. more

In this webinar, we will explore some of the latest applications for NEBuilder HiFi DNA Assembly. View full Q & A summary. more

In this video, learn how to generate single nucleosides from DNA or RNA for downstream analysis using the Nucleoside Digestion Mix. more

This video provides tips for phosphorylating fragments prior to performing a ligation reaction. more

This video provides tips for troubleshooting difficult ligation reactions and potential upstream contributing factors. more

This video explains the source of a potential white precipitate in your T4 DNA ligase buffer. more

This video explains when it's necessary to use a long incubation time for your ligation reaction. more

This video provides tips for performing an electroporation reaction following a ligation. more

This video provides tips for calculating the ideal insert:vector molar ratio before performing a ligation reaction. more

This video explains the best temperature at which to perform your ligation reaction.  Please note Hi-T4™ DNA Ligase is a thermotolerant ligase designed to function at higher temperatures than wild type T4 DNA Ligase. Hi-T4 DNA Ligase will function at temperatures as high as 50°C. more

Whether you are new to cloning or a seasoned expert, we have the webinar for you. NEB’s resident cloning experts provided an overview of various cloning methods, and answered common cloning questions received from customers. more

PCR Cloning is an easy and reliable cloning method. The name is derived from the use of a DNA amplification step to generate the amplicon. Learn more about the benefits and disadvantages of PCR Cloning. more

Dephosphorylation is the process by which phosphate groups are removed from a molecule by a phosphatase. Removal of phosphate groups from a DNA fragment can prevent ligation. Learn more about dephosphorylation and phosphatases. more

How can more efficient ligation of RNA-splinted ssDNA fragments improve your ligation-based detection assay? Learn about PBCV-1 DNA Ligase, also known as SplintR® Ligase. more

Phosphorylation is the process by which phosphate groups are added to a molecule by a kinase. The phosphorylation status of a fragment of DNA can influence its ability to proceed in reactions. Learn more about phosphorylation and kinases. more

Transformation is the process by which bacteria are made to take up exogenous DNA. The word is derived from Griffith's discovery of a "transforming principle". Learn more about transformation and how it is used in cloning workflows. more

Restriction enzymes are an integral part of the cloning workflow, for generating compatible ends on fragments and vectors. This animation discusses three guidelines for determining which restriction enzymes to use in your cloning experiment. more

The first step in determining how your ends will be blunted is to determine if they are 5´ or 3´ overhangs. This tutorial will teach you how to identify what type of overhang you have, as well as which enzyme will blunt that end, and how. more

Gibson Assembly™ employs three enzymatic activities in a single-tube reaction: 5´ exonuclease, the 3´ extension activity of a DNA polymerase and DNA ligase activity. The 5´ exonuclease activity chews back the 5´ end sequences and exposes the complementary sequence for annealing. The polymerase activity then fills in the gaps on the annealed regions. A DNA ligase then seals the nick and covalently links the DNA fragments together. The overlapping sequence of adjoining fragments is much longer than those used in Golden Gate Assembly, and therefore results in a higher percentage of correct assemblies. The NEB Gibson Assembly Master Mix (NEB #E2611) and Gibson Assembly Cloning Kit (NEB #E5510S) enable rapid assembly at 50˚C.

Polyethylene glycol (PEG) is an important reagent in ligation reactions, find out why. more

Using PCR, restriction sites are added to both ends of a dsDNA, which is then digested by the corresponding REases. The cleaved DNA can then be ligated to a plasmid vector cleaved by the same or compatible REases with T4 DNA ligase. DNA fragments can also be moved from one vector into another by digesting with REases and ligating to compatible ends of the target vector. more

The optimal reactant ratio is contingent upon the downstream application. more

Find out how the downstream application dictates the best reaction conditions for ligation. more

Ligation of blunt ends and single-base overhangs require optimized reaction conditions. more

Ligation, the process of joining DNA fragments with a DNA ligase, proceeds in three steps. Learn more about the function of ligation with our quick tutorial animation. more

This video discusses Domestication, or the removal of Type IIS cut sites naturally occurring in vector or insert sequences, as it relates to Golden Gate Assembly. more

Learn about prokaryotic argonautes, which are nucleic acid-guided endonucleases that can be used as programmable nucleases. TtAgo is the first commercially available prokaryotic argonaute.  more

Learn about endonucleases and some of their common applications. more

Learn how you can use NEBuilder HiFi to generate an sgRNA-Cas9 expression vector with a single-stranded oligo bridge. more

CRISPR systems evolved as a means of bacterial adaptive immunity. Understanding their behavior in vivo is essential to harnessing their power in vitro. more

Find out how NEBuilder® HiFi DNA Assembly can reliably join DNA fragments in a single tube, isothermal reaction, with advantages over NEB Gibson Assembly®. more

Learn how NEBuilder® HiFi DNA Assembly bridges dsDNA with a ssDNA oligo. more

Learn how to create substitutions, deletions or insertions in 3 easy steps with the Q5 Site-Directed Mutagenesis Kit. more

Tips for commonly encountered challenges in site-directed mutagenesis. more

This video gives tips on how to improve the efficiency of your DpnI digestion. more

This video explains why you might have a large number of wildtype colonies following site-directed mutagenesis and transformation. more

This video describes some things to consider if you want to incorporate multiple mutations into your plasmid. more

Watch an interactive tutorial on primer design to see how simple it really is to clone with either NEBuilder HiFi DNA Assembly or the Gibson Assembly Cloning Kit. more

Watch an interactive tutorial that details the process by which Gibson Assembly® joins DNA fragments in a single tube, isothermal reaction. more

Find out how Golden Gate Assembly can be used to quickly join multiple DNA fragments. more

In this video, see how transformation plating can be fast and easy using glass beads. more

What are toxic mini-genes, and how do they improve transformation efficiencies? Becky explains. more

Type IIS restriction enzymes have both recognition and binding sites, but cut downstream of the recognition site, creating 4-base overhangs ideal for re-assembly. View a list of Type IIS enzymes. more

Type I restriction enzymes are a group of endonucleases that recognize a bipartite sequence, but do not produce a predictable cleavage pattern. Learn more about how Type I REs work. more

Type II restriction enzymes are most commonly used for molecular biology applications, as they recognize stereotypical sequences and produce a predictable cleavage pattern. Learn more about how Type II REs work. more

Type III restriction enzymes are a group of endonucleases that recognize a non-pallindromic sequence, comprising two inversely oriented sites. Learn more about these poorly understood enzymes. more

Chromatin conformation capture (3C) techniques allow study of the spatial organization of eukaryotic chromosomes in a 3D context. more

Optical mapping is a method that allows production of restriction maps of whole chromosomes or genomes. Learn more about optical mapping.  more

Droplet digital PCR is a method for accurately quantitating copies of DNA or RNA in a sample. Each PCR reaction is separated into thousands or millions of droplets for analysis. Learn more about droplet digital PCR. more

Need a protocol to digest quickly and completely? Try this protocol for Time-Saver™ qualified enzymes from NEB. more

When cutting close to the end of a DNA molecule, make sure you know how many bases to add to the ends of your PCR primers. more

Let one of NEB's restriction enzyme experts help you improve your technique and avoid common mistakes in digest setup. more

Watch as Geoff Wilson, Restriction Enzyme Division Head, describes the interaction of restriction enzymes and substrate DNA using computer models generated from x-ray crystallography data. more

How will Time-Saver™ qualified enzymes save you time? Find out from an NEB scientist. more

Learn more about what causes this common problem, and how NEB's enzymes are QC'd to avoid DNA smearing. more

NEB has engineered HF® enzymes to eliminate star activity. Learn how, and what this means for your digests. more

Are you finding unexpected bands in your digestion reaction? Here are some tips to help you determine the cause. more

Find out how easy it is to get double digest recommendations with NEBcloner. more

More than 210 of NEB's restriction enzymes are 100% active in a single buffer. Learn more about CutSmart® Buffer and why it matters to you. more

Watch as Rick Morgan, Research Scientist in the Restriction Enzyme Division, describes his passion for discovering and characterizing restriction enzymes from nature. 
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Double digestions can save you time, and this video can offer tips for how to achieve the best results, no matter which of NEB's restriction enzymes you're using. more

Learn what Star Activity is, why it is detrimental to accurate restriction enzyme digestion, and how NEB's HF enzymes are engineered to avoid it. more

Not getting the cleavage you expected? Let an NEB scientist help you troubleshoot your reaction. more

Watch as Geoff Wilson, Restriction Enzymes Division Head, describes what restriction enzymes are and how they revolutionized molecular biology. more

Learn about endonucleases and some of their common applications. more

Type IIS restriction enzymes have both recognition and binding sites, but cut downstream of the recognition site, creating 4-base overhangs ideal for re-assembly. View a list of Type IIS enzymes. more

Learn how to do a plasmid miniprep using the Monarch Plasmid Miniprep Kit. more

Learn how you can easily recycle all of the components in your Monarch Kits. more

Learn how to extract DNA from agarose gels using the Monarch DNA Gel Extraction Kit. more

Optimize your DNA isolation from PCR and other enzymatic reactions with our quick tips for using the Monarch PCR & DNA Cleanup Kit. more

Optimize your DNA gel extractions with our quick tips for using the Monarch DNA Gel Extraction Kit. more

Learn how to isolate DNA from your enzymatic reactions, including PCR, using the Monarch PCR & DNA Cleanup Kit (5 µg). more

Optimize your plasmid miniprep results with these quick tips for using the Monarch Plasmid Miniprep Kit. more

After lysing your sample during your DNA extraction workflow, follow these tips to ensure that your purified gDNA is high-quality, intact, and free from contaminants. more

Successful genomic DNA extraction from tissues requires proper handling and storage upfront of purification. Follow our guidelines to ensure isolation of high quality, high molecular weight gDNA.
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Get helpful tips for effective lysis of tissue samples to optimize DNA extraction using the Monarch Genomic DNA Purification Kit more

Get helpful tips for DNA extraction from blood samples using the Monarch Genomic DNA Purification Kit more

This video walks you through RNA purification from a wide variety of sample types using the Monarch Total RNA Miniprep Kit. more

Learn how to use the Monarch RNA Cleanup Kits to quickly and easily purify RNA from enzymatic reactions including in vitro transcription reactions, labeling, and DNase I treatment.  more

Optimize the results of your RNA cleanups with these quick and simple tips. more

Optimize the results of your RNA extractions with these simple tips from our scientists. more

This video explains how you can shorten the length of time it takes to perform your nucleic acid cleanup. more

This video gives tips on how to avoid genomic DNA or RNA contamination when using the Monarch^® Miniprep Kits. more

This video gives tips on eluting large DNA fragments from agarose gels. more

This video gives tips for maximizing the yield when using the Monarch^® Miniprep Kits. more

This video explains the importance of placing the elution buffer in contact with the matrix for efficient elution. more

This video gives tips on adjustments you can make to your plasmid purification protocol when you're working with a large plasmid. more

This video explains why it is so important to completely melt your agarose prior to using the Monarch^® DNA Gel Extraction Kit. more

Learn how you can use NEBuilder HiFi to generate an sgRNA-Cas9 expression vector with a single-stranded oligo bridge. more

CRISPR systems evolved as a means of bacterial adaptive immunity. Understanding their behavior in vivo is essential to harnessing their power in vitro. more

Learn more about CRISPR/Cas9 and synthetic biology, where these fields are headed, and how they are being applied in the classroom in the latest episode of NEB TV. more

In this webinar you will learn how to increase editing efficiency by directly introducing Cas9 ribonucleoproteins (RNPs) to cells through electroporation or lipofection. Rapid sgRNA synthesis requiring only a single user-supplied ~55mer single-stranded DNA oligonucleotide is described. Methods for assessing genome editing efficiency will be discussed including T7-endonuclease I-based methods, sequencing-based methods, and in vitro Cas9 digestion. more

This video describes situations when your DNA should be cleaned up prior to T7 endonuclease digestion. more

This video explains why bands on a gel may not look correct after T7 digestion. more

Learn how you can use NEBuilder HiFi to generate an sgRNA-Cas9 expression vector with a single-stranded oligo bridge. more

Find out how NEBuilder® HiFi DNA Assembly can reliably join DNA fragments in a single tube, isothermal reaction, with advantages over NEB Gibson Assembly®. more

Learn how NEBuilder® HiFi DNA Assembly bridges dsDNA with a ssDNA oligo. more

Learn how to create substitutions, deletions or insertions in 3 easy steps with the Q5 Site-Directed Mutagenesis Kit. more

Tips for commonly encountered challenges in site-directed mutagenesis. more

This video gives tips on how to improve the efficiency of your DpnI digestion. more

This video explains why you might have a large number of wildtype colonies following site-directed mutagenesis and transformation. more

This video describes some things to consider if you want to incorporate multiple mutations into your plasmid. more

Watch an interactive tutorial on primer design to see how simple it really is to clone with either NEBuilder HiFi DNA Assembly or the Gibson Assembly Cloning Kit. more

Watch an interactive tutorial that details the process by which Gibson Assembly® joins DNA fragments in a single tube, isothermal reaction. more

Find out how Golden Gate Assembly can be used to quickly join multiple DNA fragments. more

Did you know that one of the advantages of NEBuilder HiFi is that it removes 3’ and 5’ -end mismatch sequences prior to fragment assembly? Learn how! more

What are toxic mini-genes, and how do they improve transformation efficiencies? Becky explains. more

Type IIS restriction enzymes have both recognition and binding sites, but cut downstream of the recognition site, creating 4-base overhangs ideal for re-assembly. View a list of Type IIS enzymes. more

Follow these tips to get superior results. more

Use this protocol for maximum transformation efficiency. more

Learn how electrocompetent cells and chemically competent cells differ in the way uptake of DNA is achieved. more

Learn whether electrocompetent cells or chemically competent cells are a better fit for your transformation. more

In this video, see how transformation plating can be fast and easy using glass beads. more

Disulfide-bonded proteins can be difficult to express, and your expression protocol may need to be optimized. Watch this quick tutorial on optimizing correctly-folded yields with SHuffle strains. more

NEB has a long history in recombinant protein expression and has developed a wide array of solutions for proteins that are difficult to express. more

What are the steps of disulfide bond formation in the periplasm, and which proteins are responsible for successful bond formation? more

What makes SHuffle cells so efficient at the expression of disulfide-bonded proteins? more

What is a disulfide bond, and how are they formed? more

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Disulfide bond formation is compartmentalized in both prokaryotes and eukaryotes. more

This webinar discusses the fundamental workflows and advantages of cell-free (in vitro) protein synthesis versus traditional cellular expression methods. more

This webinar shares solutions developed by NEB scientists for producing various classes of protein, including: genetically-tailored E.coli host strains, expression vectors and valuable guidelines for generating properly-folded recombinant protein. more

Watch this tutorial explaining the streamlined workflow for our new NEBExpress^® Cell-free Protein Synthesis System to learn how you can easily synthesize your protein in as little as 2 to 4 hours. more

A workflow animation for an enzyme-based method for detection of 5mC and 5hmC, an alternative to bisulfite sequencing. more

Behind the paper: Sriharsa and Chaithanya summarize their recent Genome Biology publication describing the NicE-seq method for open chromatin profiling and the advantages it offers over current chromatin accessibility profiling techniques. more

Curious about the role of 5-hydroxymethylcytosine in the genesis and function of the epigenome? Watch and learn as Sriharsa explains the study of 5-mC and 5-hmC as markers of epigenetically-modified genomic DNA, and the best methods for differentially detecting these modified species. more

Cancer cells have epigenetic changes to their DNA. One of these causes the turning off (silencing) of tumor suppressor genes, a small set of genes involved in cell cycle control. Sriharsa discusses drugs currently in the clinic that are able to demethylate DNA and improve the lives of cancer patients. more

Watch an interactive tutorial explaining the different molecular mechanisms by which epigenetic change influences gene expression. Learn about how NEB’s reagents are targeted to the various enzymes and DNA elements that are altered by epigenetic change. more

Follow NEB Product Development Scientist, Romas Vaisvila, Ph.D., as he demonstrates the EpiMark® 5-mc and 5-hmc Analysis Kit for locus specific identification and quantification of 5-hmc in this protocol "High Sensitivity 5-hmc detection in Balb/C Brain Tissue".

Epigenetic changes to DNA impact a wide range of biological processes such as organism development and disease progression. Watch as Sriharsa enthusiastically describes this exciting and complex field that has the power to "re-write" the course of DNA transcription. more

New England Biolabs is pleased to introduce the EpiMark® 5-hmC and 5-mC Analysis Kit, a simple and robust method for the identification and quantitation of 5-hydroxymethylcytosine (5-hmC) and 5-methylcytosine (5-mC) within a specific DNA locus.

EpiMark® is a registered trademark of New England Biolabs, Inc.

Rick Feehery, Research Associate at New England Biolabs, Inc, explains the mechanisms of epigenetic DNA modification. To drive home the power of such tiny changes, he uses the example of honeybee colonies, wherein DNA methylation is the only factor that determines whether a bee is born a worker, drone or queen. more

If all cells are created from the same genetic material, why are there so many different cell types? Listen to Sriharsa Pradhan, Senior Scientist, RNA Biology at NEB, as he describes how DNA is methylated and how this affects the path of reading the DNA code the same way an obstruction would derail a train off its tracks. more

NEB and NIBRT scientists devised a robust workflow for comprehensive profiling of GSL head group glycans, which has the potential to facilitate functional glycomics studies and biomarker discovery. Chris shares details of this recent publication. more

Minyong and Jim summarize their recent Nature Communications publication describing selective capture of N-glycans and N-glycopeptides by an engineered high affinity Fbs1 carbohydrate binding protein. more

This two-step deglycosylation protocol is for use with New England Biolabs’ Rapid PNGase F. Learn more about Rapid PNGase F.  more

This one-step deglycosylation protocol is for use with New England Biolabs’ Rapid PNGase F. Learn more about Rapid PNGase F. more

Learn about glycobiology and its importance in clinical and diagnostic applications in this episode of NEB TV. Also, hear more about how NEB is setting the bar for product quality in this rapidly growing field. more

Isolating, by biopanning, the phage that binds to bone allows researchers to identify the peptide sequences that stimulate the differentiation of mesenchymal cells and potentiate bone repair. more

Learn about the core sequences and common modifications of N-linked and O-linked glycans in this video. Analysis of these glycans and/or peptide portions of the glycoprotein can be accomplished with the use of deglycosylation enzymes, which are explained in detail. Unlike other chemical deglycosylation methods, enzymatic treatment is much gentler and can provide complete sugar removal with no protein degradation. more

Learn more about the structural elucidation of complex and diverse heparin oligosaccharides using Bacteroides Heparinase I, II and III in combination with downstream mass spec analysis. more

Here we illustrate the use of glycosidases for the analysis of a model glycoprotein: recombinant human chorionic gonadotropin beta (hCGβ), which carries both N-glycans and O-glycans in this video. The technique requires only simple instrumentation and typical consumables, and it can be readily adapted to the analysis of multiple glycoprotein samples. more

Learn the basics of how large DNA molecules are separated with Pulsed Field Gel Electrophoresis (PFGE). more

In this video, you will learn how to prepare a pulsed field gel marker and load it onto an agarose gel. more

In this video, you will learn how to prepare a standard DNA ladder and load it onto an agarose gel. more

Learn more about Cappable-seq and SMRT Cappable-seq, and how they can be used for prokaryotic transcription start sites (TSS) determination. more

Find out how the Nucleoside Digestion Mix can be used to for the identification and quantitation of DNA and RNA modifications. more

Learn about the sources of RNase contamination and how you can prevent them from disrupting your experiments. more

In this episode we are talking about the importance of RNA modifications. Hear about protocols for prokaryotic transcription start site (TSS) determination, as well as detection and quantitation of RNA modifications, and learn how the RNA Modification Database can help your RNA research. more

Behind the paper: Jennifer summarizes her group’s recent Nucleic Acid Research publication describing how individual RNA base modifications affect RNA polymerase fidelity as well as reverse transcription error rates. more

This method describes high yield in vitro synthesis of both capped and uncapped mRNA from a linearized plasmid containing the Gaussia luciferase (GLuc) gene. The RNA is purified and a fraction of the uncapped RNA is enzymatically capped using the Vaccinia virus capping enzyme. In the final step, the mRNA is transfected into HeLa cells and cell culture supernatants are assayed for luciferase activity. more

View an interactive tutorial explaining the mechanism of our SNAP-tag® technologies and reagents available for researchers wishing to study the function and localization of proteins in live or fixed cells. more

Watch as Chris Provost, of New England Biolabs, performs fluorescent imaging of live COS-7 cells expressing SNAP-tag^® fusion proteins. more