Customers frequently ask “Why is there a white precipitate in my T4 DNA ligase buffer?”
If you see a white precipitate in your T4 DNA ligase buffer it is most likely DTT falling out of solution. It is important not to heat the buffer to resuspend the precipitate, as this will degrade the ATP, which is necessary for ligation. DTT is found in excess in the buffer and the small amount lost in the precipitation will not affect your ligation efficiency.
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