A lot of you have been asking me whether to do a size selection or a simple cleanup for the NEBNext Ultra DNA or RNA workflows.
The first consideration would be whether you need a precise insert range. If you need a precise insert range, you would have to do a size selection.
The other factor is, how much starting material you have. If your starting material is low and you are doing a size selection you could potentially reduce the complexity of your library. So, if your input amount is low, just do a cleanup.
It is always important to remove adaptor dimer. If you have adaptor dimer after the PCR cleanup, you can do another 0.9x SPRI bead ratio cleanup.
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