Customers often ask us how they can maximize yield when using our Monarch Miniprep Kit. Here are a few helpful tips.
Resuspend your pellet completely. Any bacteria that haven't been resuspended won't lyse efficiently, and the amount of DNA that you will obtain will be lower.
When lysing the cells, invert the tubes several times so that the solution becomes viscous and uniformly darker pink.
Make sure that you add the elution buffer to the center of the matrix without puncturing it. The elution buffer needs to be in contact with the column matrix for efficient elution. Be sure to incubate the elution buffer in the column for a full minute before spinning.
Remember, if you have any additional questions, you can always contact our tech support team at firstname.lastname@example.org.
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