• My NEB
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  • Icon Descriptions

    The gene encoding this enzyme has been cloned at New England Biolabs, Inc.

    This enzyme is purified from a recombinant source.

    This enzyme has been engineered.

    This enzyme will digest unit substrate in 5-15 minutes under recommended reaction conditions, and can also be used safely in overnight digestions.

    Indicates which reaction NEBuffer is supplied with the enzyme for optimal activity. Enzymes with buffer requirements not met by one of the four standard NEBuffers (1.1, 2.1, 3.1 or CutSmart) are supplied with their own unique NEBuffer (NEBuffer U). NEBuffers are color-coded (NEBuffer 1.1-yellow, NEBuffer 2.1-blue, NEBuffer 3.1-red, CutSmart-green) and supplied as 10X stocks with each enzyme. For more information, consult the NEBuffer Performance Chart.

    Indicates the product can amplify DNA directly with no need for extraction.

    Indicates the enzyme's optimum incubation temperature.

    This enzyme is supplied with a separate tube of S-adenosylmethionine (SAM). To obtain 100% activity SAM should be added to the 1X reaction mix as indicated. When required, a concentrated stock of SAM is supplied with the enzyme.

    Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by either the dam or dcm or CpG methylase.

    Indicates whether or not the enzyme can be heat inactivated. Enzymes are first tested by incubation at 65°C for 20 minutes; any enzyme not inactivated at 65°C is then tested by incubation at 80°C for 20 minutes.

    This enzyme has passed the blue/white selection assay. This assay is performed on enzymes with single sites in cloning vectors, it is an extremely sensitive measure of the integrity of DNA fragment ends after excess digestion with the enzyme.