Icon Descriptions

The gene encoding this enzyme has been cloned at New England Biolabs, Inc.

This enzyme is purified from a recombinant source.

This enzyme has been engineered.

This enzyme will digest unit substrate in 5-15 minutes under recommended reaction conditions, and can also be used safely in overnight digestions.

This enzyme has reduced star activity. 

Epimark-validated enzyme for epigenetic studies.

Indicates that the polymerase is high fidelity.

Indicates that the polymerase is a hot start/warm start enzyme.

This polymerase is used for PCR.

Ta = Tm + 3°C for the polymerase.

Ta = Tm – 5°C for the polymerase.

Indicates which reaction NEBuffer is supplied with the enzyme for optimal activity. Enzymes with buffer requirements not met by one of the four standard NEBuffers (1.1, 2.1, 3.1 or CutSmart) are supplied with their own unique NEBuffer (NEBuffer U). NEBuffers are color-coded (NEBuffer 1.1-yellow, NEBuffer 2.1-blue, NEBuffer 3.1-red, CutSmart-green) and supplied as 10X stocks with each enzyme. For more information, consult the NEBuffer Performance Chart.

extraction free
Indicates the product can amplify DNA directly with no need for extraction.

incubation temp incubation temp
Indicates the enzyme's optimum incubation temperature.

Indicates that the restriction enzyme requires two or more sites for cleavage. 

This enzyme is supplied with a separate tube of S-adenosylmethionine (SAM). To obtain 100% activity SAM should be added to the 1X reaction mix as indicated. When required, a concentrated stock of SAM is supplied with the enzyme.

This enzyme is supplied with a separate tube of bovine serum albumin (BSA). To obtain 100% activity BSA should be added to the 1X reaction mix to a final concentration of 100 µg/ml. When required, BSA is supplied as a 100X concentrated stock (10 mg/ml). 

dam dcm cpg
Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by either the dam or dcm or CpG methylase.

heat inactivation yes heat inactivation no heat inactivation heat inactivation
Indicates whether or not the enzyme can be heat inactivated. Enzymes are first tested by incubation at 65°C for 20 minutes; any enzyme not inactivated at 65°C is then tested by incubation at 80°C for 20 minutes.

blue white
This enzyme has passed the blue/white selection assay. This assay is performed on enzymes with single sites in cloning vectors, it is an extremely sensitive measure of the integrity of DNA fragment ends after excess digestion with the enzyme.