The gene encoding this enzyme has been cloned at New England Biolabs, Inc. This enzyme is purified from a recombinant source. This enzyme has been engineered. This enzyme is capable of digesting 1 µg of DNA in 5-15 minutes. This enzyme has reduced star activity. Indicates which reaction NEBuffer is supplied with the enzyme for optimal activity. Enzymes with buffer requirements not met by one of the four standard NEBuffers (1, 2, 3 or 4) are supplied with their own unique NEBuffer (NEBuffer U). NEBuffers are color-coded (NEBuffer 1-yellow, NEBuffer 2-blue, NEBuffer 3-red, NEBuffer 4-green) and supplied as 10X stocks with each enzyme. For more information, consult the NEBuffer Activity Chart. This enzyme is supplied with a separate tube of bovine serum albumin (BSA). To obtain 100% activity BSA should be added to the 1X reaction mix to a final concentration of 100 µg/ml. When required, BSA is supplied as a 100X concentrated stock (10 mg/ml).