Home Tools & Resources Selection Charts DNA Markers & Ladders Selection Chart

DNA Markers & Ladders Selection Chart

NEB offers a variety of DNA ladders with sizes ranging from 10 bp to 48.5 kb for use in DNA gel electrophoresis, including standard agarose and polyacrylamide (PAGE) electrophoresis. NEB also offers various conventional double-stranded DNA molecular weight markers with sizes up to 23 kb and markers for pulsed-field gel electrophoresis (PFGE) ranging from 1.5 to 1,018 kb. NEB DNA ladders are available in standard formats and in a variety of convenient, ready-to-load formats.


  NEB # Gel Lanes Size Range Ready to Load? Supplied with Loading Dye? Compatible with Standard Electrophoresis* Other Applications
1 kb Plus
1 kb Plus DNA Ladder N3200S
N3200L		 200
1,000		 100 bp–10 kb – ✔ ✔  
Quick-Load Purple 1 kb Plus DNA Ladder N0550S
N0550L		 250
750		 100 bp–10 kb ✔ ✔ ✔  
Quick-Load 1 kb Plus DNA Ladder N0469S 250 100 bp–10 kb ✔ – ✔  
1 kb Plus DNA Ladder for Safe Stains N0559S 250 100 bp–10 kb ✔
 –
 ✔
Optimized for GelRed®, GelGreen®, SYBR® pre-cast gels		  
Tri-Dye 1 Kb Plus DNA Ladder N3270S 250 100 bp–10 kb ✔
 – ✔  
1 kb Extend
Quick-Load 1 kb Extend DNA Ladder N3239S 125 500 bp–48.5 kb ✔ – ✔  
100 bp
100 bp DNA Ladder N3231S
N3231L		 100
500 		100–1,517 bp – ✔ ✔  
Quick-Load Purple 100 bp DNA Ladder N0551S
N0551L 		125
375 		100–1,517 bp ✔ ✔ ✔  
Quick-Load 100 bp DNA Ladder N0467S
N0467L 		125
375 		100–1,517 bp ✔
 – ✔  
TriDye 100 bp DNA Ladder N3271S 125 100–1,517 bp ✔ – ✔  
1 kb
1 kb DNA Ladder N3232S
N3232L

200
1,000

 500 bp–10 kb
 –
 ✔
 ✔  
Quick-Load Purple 1 kb DNA Ladder N0552S
N0552L		 125
375 		500 bp–10 kb ✔ ✔ ✔  
Quick-Load 1 kb DNA Ladder N0468S
N0468L 		125
375 		500 bp–10 kb ✔
 – ✔  
TriDye 1 kb DNA Ladder N3272S 125 500 bp–10 kb ✔ – ✔  
50 bp
50 bp DNA Ladder N3236S
N3236L		 200
1,000 		50–1,350 bp – ✔ ✔  
Quick-Load Purple 50 bp DNA Ladder N0556S 250 50–1,350 bp ✔ ✔ ✔  
Low Molecular Weight
Low Molecular Weight DNA Ladder N3233S
N3233L		 100
500 		25–766 bp – ✔ ✔  
Quick-Load Purple Low Molecular Weight DNA Ladder N0557S 125 25–766 bp ✔ ✔ ✔  
TriDye™ Ultra Low Range DNA Ladder N0558S 250 10–700 bp ✔
 –
 ✔ Polyacrylamide Gel Electrophoresis (PAGE)
Supercoiled
Supercoiled DNA Ladder N0472S 100 2–10 kb – ✔ ✔  
Fast DNA Ladder
Fast DNA Ladder N3238S 50 50 bp–10 kb ✔
Xylene Cyanol FF Loading Buffer

 

 – ✔
Compatible with GelRed, GelGreen, SYBR pre-cast gels		 Fast Gel Electrophoresis (e.g., E-Gels)
PCR Marker
PCR Marker N3234S
N3234L 		100
500 		50–766 bp – ✔ ✔  
Conventional Markers
pBR322 DNA-BstNI Digest N3031L 250 13–1,857 bp – ✔ ✔  
λ DNA-HindIII Digest N3012S
N3012L		 150
750 		125 bp–23.1 kb – ✔ ✔  
λ DNA-BstEII Digest N3014S 150 117–8,454 bp – ✔ ✔  
ΦX174 DNA-HaeIII Digest N3026S
N3026L 		50
250 		72–1,353 bp – ✔ ✔  
pBR322 DNA-MspI Digest N3032S  50
9–622 bp – ✔ ✔  
Pulsed Field Gel (PFG) Markers
λ DNA-Mono Cut Mix N3019S 100 1.5–48.5 kb – ✔ ✔ Pulsed Field Gel Electrophoresis (PFGE)
λ PFG Ladder N0341S 50 48.5–1,018.5 kb Solid Agarose – – Pulsed Field Gel Electrophoresis (PFGE)
MidRange PFG Marker N0342S 50 15–291 kb Solid Agarose – – Pulsed Field Gel Electrophoresis (PFGE)

*Not optimized for gels pre-cast with GelRed®, GelGreen®, SYBR® dyes unless otherwise stated.

Tracking Dyes
Quick-Load = Bromophenol Blue
Quick-Load Purple = Purple
TriDye = Bromophenol Blue, Xylene Cyanol FF, Orange G