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Publications

We believe that basic research and the cultivation of scientific knowledge is critical for us to stay connected with our customers and to drive scientific breakthroughs. At NEB, over 30 labs participate in research projects, which are aided by post-doctoral fellows and students in Masters and Ph.D. programs. NEB researchers have authored or co-authored over 1,200 publications (as of 1/19) many of which are in peer-reviewed journals. Further, NEB products have been used successfully in numerous publications by scientists throughout the world.

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Year Title Journal Authors Application(s)
2014 A versatile system for USER cloning-based assembly of expression vectors for mammalian cell engineering PLoS One Lund AM, Kildegaard HF, Petersen MB, Rank J, Hansen BG, Andersen MR, Mortensen UH USER™ Cloning
2014 Rapid and Efficient Assembly of Transcription Activator-Like Effector Genes by USER Cloning J Genet Genomics Wang S, Li W, Wang S, Hu B USER™ Cloning
2010 USER friendly DNA recombination (USERec): a simple and flexible near homology-independent method for gene library construction Protein Eng Des Sel Villiers BR, Stein V, Hollfelder F USER™ Cloning
2019 Fusion to Tetrahymena thermophila granule lattice protein 1 confers solubility to sexual stage malaria antigens in Escherichia coli. Protein Expression and Purification Agrawal, A., Bisharyan, Y., Papoyan, A, Bednenko, J., Cardarelli, J., Yao, M., Clark, T., Berkm​en, M., Ke, N., Colussi, P. Protein Expression,Protein Expression in E. Coli,Expression of Difficult Proteins,T7 Expression,Non-T7 Expression,Disulfide-bonded Protein Expression,Target Protein Insolubility
2019 Characterization of the internal translation initiation region in monoclonal antibodies expressed in Escherichia coli Journal of Biological Chemistry Leith, E.M., O'Dell, W.B., Ke, N., McClung, C., Berkmen, M., Bergonzo, C., Brinson, R.G., Kelman, Z Protein Analysis & Tools,Protein Expression,Proteomics,Protein Expression in E. Coli,Expression of Difficult Proteins,T7 Expression,Disulfide-bonded Protein Expression
2015 Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria Nature Communications Robinson, M.-P., Ke, N., Lobstein, J., Peterson, C., Szkodny, A., Mansell, T.J., Tuckey, C., Riggs, P.D., Colussi, P.A., Noren, C.J., Taron, C.H., Delisa, M.P., Berkmen, M. Protein Expression,Protein Expression in E. Coli,Expression of Difficult Proteins,T7 Expression,Disulfide-bonded Protein Expression
2018 Platform development for expression and purification of stable isotope labeled monoclonal antibodies in Escherichia coli. mAbs Mabs Reddy, P.T., Brinson, R.G., Hoopes, J.T., McClung, C., Ke, N., Kashi, L. Protein Expression,Protein Expression in E. Coli,Expression of Difficult Proteins,T7 Expression,Disulfide-bonded Protein Expression
2019 Non templated addition and template switching by Moloney murine leukemia virus MMLV based reverse transcriptases co occur and compete with each other J. Biol. Chem Wulf, M.G., Maguire, S., Humbert, P., Dai, N., Bei, Y., Nichols, N.M., Correa, I.R., Jr., Guan, S RNA Analysis,RNA-seq,RT-qPCR, RT-PCR and cDNA Synthesis,Small RNA Detection and Isolation
2015 Iron-induced Local Complement Component 3 (C3) Up-regulation via Non-canonical Transforming Growth Factor (TGF)-β Signaling in the Retinal Pigment Epithelium. J Biol Chem Yafeng Li, Delu Song, Ying Song, Liangliang Zhao, Natalie Wolkow, John W Tobias, Wenchao Song, Joshua L Dunaief Site Directed Mutagenesis,High-Fidelity PCR
2019 A ligand based system for receptor specific delivery of proteins Sci Rep Maffei, M., Morelli, C., Graham, E., Patriarca, S., Donzelli, L., Doleschall, B., de Castro, Reis, F., Nocchi, L., Chadick, C.H., Reymond, L., Correa, I.R., Jr., Johnsson, K., Hackett, J.A., Heppenstall, P.A Cellular Analysis,Cell Imaging,In vivo Imaging,CLIP Surface,SNAP Surface