Enzymes For Innovation

The NEB catalog highlights a wide variety of enzyme functionalities found in nature or engineered for specific purposes. However, in molecular biology, new tools can often lead to new discoveries. Taking advantage of the enzymology expertise at NEB, we are offering novel enzymes with interesting and unique activities for manipulating DNA, RNA, proteins and glycans, even if specific applications for them have yet to be discovered. Our hope is that by engaging researchers' imaginations, our “Enzymes for Innovation” initiative will enable the development of new molecular techniques that so often lead to new discoveries.

Products Available

5-hydroxymethyluridine DNA Kinase M0659 5-HMUDK mechanism

5-hydroxymethyluridine DNA Kinase (5-HMUDK) transfers the gamma phosphate from ATP to the hydroxymethyl moiety of 5-hydroxymethyluridine in polymeric DNA.
EcoGII Methyltransferase M0603 EFI_EcoGII_Methyltransferase

EcoGII Methyltransferase is a non-specific methyltransferase that modifies adenine residues (N6) in any sequence context.
Endoglycoceramidase I (EGCase I) P0773 EFI_EGCaseI

Catalyzes the hydrolysis of the β-glycosidic linkage between oligosaccharides and ceramides in various glycosphingolipids.
Nb.BssSI R0681 EFI_NbBssSI

Generates a nick in the 3´ strand at the following recognition site: CACGAG (none/-1).
NudC Pyrophosphatase M0607


NudC is a NUDIX pyrophosphatase that efficiently hydrolyzes NAD+ - and NADH-capped RNA, generating a ligatible 5´ monophosphate on the RNA (NAD+ decapping or deNADding).

RtcB Ligase M0458 EFI_RtcBligase

Joins single-stranded RNA with a 3´-phosphate or 2´,3´-cyclic phosphate to another RNA with a 5´ -hydroxyl. 
TelN Protelomerase M0651  EFI_TelN_Protelomerase

Cuts dsDNA at a TelN recognition sequence (56 bp) and leaves covalently closed ends at the site of cleavage.
Thermostable FEN1 M0645  EFI_ThermostableFEN1

Catalyzes the cleavage of 5´ DNA flaps from branched double-stranded DNA substrates.
Tte UvrD Helicase M1202

Tte UvrD Helicase

Tte UvrD Helicase is a repair helicase capable of unwinding double-stranded DNA, without a requirement for a specific flap or overhang structure, from the thermophilic organism Thermoanaerobacter tengcongensis.

 Tth Argonaute  M0665


TtAgo is a prokaryotic argonaute that functions as a DNA-guided endonuclease when provided with a 16–18 nucleotide long 5´-phosphorylated single-stranded DNA oligonucleotide guide.

If you are looking for an enzyme functionality that is not currently available, contact [email protected].

Have you found a good application for one of the enzymes listed above? we would love to hear about it!

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