The NEB catalog highlights a wide variety of enzyme functionalities found in nature or engineered for specific purposes. However, in molecular biology, new tools can often lead to new discoveries. Taking advantage of the enzymology expertise at NEB, we are offering novel enzymes with interesting and unique activities for manipulating DNA, RNA, proteins and glycans, even if specific applications for them have yet to be discovered. Our hope is that by engaging researchers' imaginations, our “Enzymes for Innovation” initiative will enable the development of new molecular techniques that so often lead to new discoveries.
|5-hydroxymethyluridine DNA Kinase||M0659||
5-hydroxymethyluridine DNA Kinase (5-HMUDK) transfers the gamma phosphate from ATP to the hydroxymethyl moiety of 5-hydroxymethyluridine in polymeric DNA.
EcoGII Methyltransferase is a non-specific methyltransferase that modifies adenine residues (N6) in any sequence context.
|Endoglycoceramidase I (EGCase I)||P0773||
Catalyzes the hydrolysis of the β-glycosidic linkage between oligosaccharides and ceramides in various glycosphingolipids.
Generates a nick in the 3´ strand at the following recognition site: CACGAG (none/-1).
NudC is a NUDIX pyrophosphatase that efficiently hydrolyzes NAD+ - and NADH-capped RNA, generating a ligatible 5´ monophosphate on the RNA (NAD+ decapping or deNADding).
Joins single-stranded RNA with a 3´-phosphate or 2´,3´-cyclic phosphate to another RNA with a 5´ -hydroxyl.
Cuts dsDNA at a TelN recognition sequence (56 bp) and leaves covalently closed ends at the site of cleavage.
Catalyzes the cleavage of 5´ DNA flaps from branched double-stranded DNA substrates.
|Tte UvrD Helicase||M1202||
Tte UvrD Helicase is a repair helicase capable of unwinding double-stranded DNA, without a requirement for a specific flap or overhang structure, from the thermophilic organism Thermoanaerobacter tengcongensis.
TtAgo is a prokaryotic argonaute that functions as a DNA-guided endonuclease when provided with a 16–18 nucleotide long 5´-phosphorylated single-stranded DNA oligonucleotide guide.
If you are looking for an enzyme functionality that is not currently available, contact [email protected].
Have you found a good application for one of the enzymes listed above? we would love to hear about it!