Where can I find guidelines and protocols for using the NEBNext ARTIC SARS-CoV-2 FS Library Prep Kit, including cDNA synthesis, targeted cDNA amplification, end repair and ligation?
Protocols for use of NEB #E7660 can be found within the product manual, including:
- cDNA Synthesis
- Targeted cDNA Amplification
- Cleanup of targeted cDNA amplicons
- NEBNext End Prep
- Barcode Ligation
- Cleanup of Barcode-ligation DNA
- Adapter Ligation
- Cleanup of Adapter Ligated DNA
Protocols.io also provides an interactive version of this protocol where you can discover and share optimizations with the research community.