Where can I find guidelines and protocols for using the NEBNext ARTIC SARS-CoV-2 FS Library Prep Kit, including cDNA synthesis, targeted cDNA amplification, end repair and ligation, and the Express protocol option with reduced cleanup steps?

Protocols for use of NEB #E7660 can be found within the product manual, including:

  • cDNA Synthesis 
  • Targeted cDNA Amplification 
  • Cleanup of targeted cDNA amplicons (Standard protocol only)
  • NEBNext End Prep 
  • Barcode Ligation 
  • Cleanup of Barcode-ligation DNA 
  • Adapter Ligation 
  • Cleanup of Adapter Ligated DNA 

Protocols.io also provides an interactive versions of the Standard protocol and the Express protocol, where you can discover and share optimizations with the research community.