Where can I find guidelines and protocols for using the NEBNext ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies), including cDNA synthesis, targeted cDNA amplification, end repair and ligation, and the Express protocol option with reduced cleanup steps?

Protocols for use of NEB #E7660 can be found within the product manual. The current manual includes VarSkip Short v2 primers and NEBNext ARTIC primers. The earlier manual includes the original VarSkip Short primers and NEBNext ARTIC primers.

Both manuals include:

  • cDNA Synthesis 
  • Targeted cDNA Amplification 
  • Cleanup of targeted cDNA amplicons (Standard protocol only)
  • NEBNext End Prep 
  • Barcode Ligation 
  • Cleanup of Barcode-ligation DNA 
  • Adapter Ligation 
  • Cleanup of Adapter Ligated DNA 

Protocols.io also provides interactive versions of protocols for NEBNext ARTIC in the NEBNext ARTIC Protocols Collection, where you can discover and share optimizations with the research community.