Protocol for RecJf (NEB #M0264)
RecJf is a single-stranded DNA specific exonuclease that catalyzes the removal of deoxynucleotide monophosphates from DNA in the 5´ → 3´ direction.
- Set-up a typical reaction as follows:
|COMPONENTS||50 μl REACTION|
|DNA||Up to 1 µg|
|NEBuffer 2 (10X)||5 µl (1X)|
|RecJf||1 µl (30 units)|
|Nuclease-free H2O (NEB #B1500S)||up to 50 µl|
- Incubate at 37°C for 20 minutes.