Enzymatic PCR Cleanup Protocol (NEB #M0525)
Enzymatic PCR Cleanup Protocol
- Set-up the reaction as follows:
- Incubate the mix at the 37°C for 15 minutes.
- Inactivate enzymes at 80°C for 15 minutes.
|PCR Product||5 µl|
|Exonuclease I (M0293)||0.5 µl (10 units)|
|Quick CIP (NEB #M0525)||1 µl (5 units)|
*[Note that the same volume of Shrimp Alkaline Phosphatase (rSAP)* (NEB #M0371) can be substituted for Quick CIP.]
PCR products are ready for downstream application.
Interested in a 5-minute protocol? Try out the Exo-CIP™ Rapid PCR Cleanup Kit (NEB #E1050)