Protocol for Exo-CIP™ Rapid PCR Cleanup (#E1050)

  • Transfer 5 µl of PCR product to a new PCR tube and add 1 µl of Exo-CIP A and Exo-CIP B respectively. The final volume is 7 µl. 

  • Mix thoroughly and briefly centrifuge at 1000 x g.

  • Incubate the reaction tube for 4 minutes at 37°C followed by 1 minute at 80°C.

  • Submit 3 µl or less (in a range of 15-200 fmol)* of treated PCR product for sequencing using BigDye™ Terminator v3.1 Cycle Sequencing Kit or store the treated samples at -20°C for longer term storage.

* A simple way to determine the amount of your amplicon is to load 3 µl on an agarose gel along with a known amount of a control DNA for comparison. Alternatively, direct measurement using fluorescent dye based kit (e.g., Qubit™) will ensure the proper amount of DNA is submitted.

Size of PCR amplicon ng of DNA (in 3 ul sample)
100 bp 1 - 12
500 bp 5 - 60
1000 bp 10 - 120
3000 bp 30 - 360
5000 bp 50 - 600