Protocol for T4 PDG (M0308)
1) Reaction set-up:
|T4 PDG Reaction Buffer (10X)||2-5 µl|
|T4 PDG (T4 Endonuclease V)||1 µl (10 units)|
|H2O||Up to 20-50 µl|
2) Incubate at 37°C for 30 minutes.
Addition of SDS to a final concentration of 0.5%, or 1µl phenol, in 6X Loading dye to the sample before running the gel will improve electrophoresis by dissociating the protein from the DNA.