Protocol for T5 Exonuclease (M0363)
|DNA||x µl (1 µg)|
|10X NEBuffer 4||5 µl|
|T5 Exonuclease||1 µl (10 units)|
|H2O||x µl (up to 50 µl)|
- Incubate at 37°C for 30 minutes.
- To stop reaction, add EDTA to a concentration of 11 mM.
- Clean-up treated samples by column purification or phenol/chloroform extraction followed by ethanol precipitation.