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  • A-Tailing with Terminal Transferase

    1. Set-up the reaction as follows:

    • PCR-amplified DNA – X
    • 10X Terminal Transferase Reaction Buffer – 7.5ul
    • 1 mM ddATP – 1.5 ul
    • CoCl2 – 7.5 ul
    • Terminal Transferase – 6 ul
    • H2O – X
    Total Reaction Volume – 75 ul (This volume can be adjusted based on the volume of PCR-amplified DNA that needs to be added).

    2. Incubate the reaction at 37 oC for 1.5 hr.