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  • Assess library quality on a Bioanalyzer® (Agilent high sensitivity chip) (E7530)

    Protocol

    1. Dilute (1:4) library in nuclease-free water.

    2. Run 1 μl in a DNA High Sensitivity chip.

    3. Check that the electropherogram shows a narrow distribution with a peak size approximately 300 bp.
    Note: If a peak at ~ 80 bp (primers) or 128 bp (adaptor-dimer) is shown in the bioanalyzer traces; Bring up the sample volume to 50 μl exactly with nuclease-free water and repeat the AMPure XP bead clean up step.

    Example of RNA library size distribution on a Bioanalyzer.