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  • Perform Second Strand cDNA Synthesis (E7420)

    Protocol

    1. Add the following reagents to the First Strand Synthesis reaction (20 μl):

      Nuclease-free Water   48 μl 
      Second Strand Synthesis Reaction Buffer(10X)   8 μl 
      Second Strand Synthesis Enzyme Mix   4 μl
      ------------------------------------------------------------
      Total volume   80 μl

    2. Mix thoroughly by gentle pipetting.

    3. Incubate in a thermal cycler for 1 hour at 16°C, with heated lid set at ≤ 40°C.