- Add 50 μl of 0.2 M glycine (pH 2.5) to the bead pellet, vortex and incubate for 5 minutes at 4°C with agitation.
- Apply magnetic field and transfer eluted IgG to clean microcentrifuge tube.
- Add an additional 50 μl of 0.2 M glycine to the beads and repeat elution step. Pool elution supernatants and neutralize by adding 20 μl of 1 M Tris-HCl (pH 9.0). Store at 4°C.