Carrier Protein 27 (Western) Protocol 1: For Western Blotting
- Dissolve your peptide in water to a concentration of 1 mM.
- Thaw the carrier protein at room temperature.
- Mix the following (in order):
- Incubate at room temperature for 15-60 minutes or at 4°C overnight.
- Add 12.5 µl of 3X SDS-PAGE Sample Buffer.
- Heat at 95°C for 5 minutes.
- Load 5-10 µl/lane (~25-50 ng) for Western blotting.