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SNAP-Capture products are non-magnetic or magnetic agarose beads coupled to a benzylguanine substrate, used to selectively capture and immobilize SNAP-tag® fusion proteins from solution. These beads have a high loading capacity for SNAP-tag fusion proteins and show very low non-specific absorption of proteins from a complex lysate, making them suitable for pull-down applications.

SNAP-tag® is a registered trademark of New England Biolabs, Inc.

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    Publications related to SNAP-Capture
    • Kindermann M. et al. (2003) Covalent and selective immobilization of fusion proteins J Am Chem Soc; 125, 7810-7811 . PubMedID: 12822993
    • Maffei, M., Morelli, C., Graham, E., Patriarca, S., Donzelli, L., Doleschall, B., de Castro, Reis, F., Nocchi, L., Chadick, C.H., Reymond, L., Correa, I.R., Jr., Johnsson, K., Hackett, J.A., Heppenstall, P.A (2019) A ligand based system for receptor specific delivery of proteins Sci Rep; 9(1), 19214.. PubMedID: 31844114, DOI: 10.1038/s41598-019-55797-1
    • Jongsma M.A., Litjens R. H. (2006) Self-assembling protein arrays on DNA chips by auto-labeling fusion proteins with a single DNA address Proteomics; 6, 2650-2655 . PubMedID: 16596705
    • Sielaff I. et al. (2006) Protein function microarrays based on self-immobilizing and self-labeling fusion proteins Chembiochem; 7, 194-202. PubMedID: 16342318
    • Kufer S.K. et al. (2005) Covalent immobilization of recombinant fusion proteins with hAGT for single molecule force spectroscopy Eur Biophys J; 35, 72-78. PubMedID: 16160825
    • Tugulu S. et al. (2005) Protein-functionalized polymer brushes Biomacromolecules; 6, 1602-1607. PubMedID: 15877383
    • Engin S. et al. (2010) Benzylguanine Thiol self-assembled monolayers for the immobilization of SNAP-tag proteins on microcontact-printed surface structures Langmuir; ASAP, PubMedID: 20369837
    • Iversen L. et al. (2008) Templated protein assembly on micro-contact-printed surface patterns. Use of the SNAP-tag protein functionality Langmuir; May 17, PubMedID: 18484753
    • Huber W. et al. (2004) SPR-based interaction studies with small molecular weight ligands using hAGT fusion proteins Anal Biochem; 333, 280-288 . PubMedID: 15450803
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While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please email us at busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.