SNAP-Capture products are agarose or magnetic cellulose beads coupled to a benzylguanine substrate, used to selectively capture and immobilize SNAP-tag® fusion proteins from solution. These beads have a high loading capacity for SNAP-tag fusion proteins and show very low non-specific absorption of proteins from a complex lysate, making them suitable for pull-down applications.
SNAP-tag® is a registered trademark of New England Biolabs, Inc.
FAQs for SNAP-Capture
Protocols for SNAP-Capture
Cellular Imaging and Analysis Brochure
The Cellular Imaging and Analysis brochure provides information on the labeling technologies offered by NEB for studying the function and localization of proteins in cells.
Other Tools & Resources
- Kindermann M. et al. 2003. Covalent and selective immobilization of fusion proteins JACS . 125, PubMedID: 12822993, DOI:
- Huber W. et al. 2004. SPR-based interaction studies with small molecular weight ligands using hAGT fusion proteins Anal. Biochem. . 333, PubMedID: 15450803, DOI:
- Sielaff I. et al. 2006. Protein function microarrays based on self-immobilizing and self-labeling fusion proteins ChemBioChem.. 7, PubMedID: 16342318, DOI:
- Jongsma M.A., Litjens R. H. 2006. Self-assembling protein arrays on DNA chips by auto-labeling fusion proteins with a single DNA address Proteomics . 6, PubMedID: 16596705, DOI:
- Tugulu S. et al. 2005. Protein-functionalized polymer brushes Biomacromolecules . 6, PubMedID: 15877383, DOI:
- Kufer S.K. et al. 2005. Covalent immobilization of recombinant fusion proteins with hAGT for single molecule force spectroscopy Eur. Biophys. J . 35, PubMedID: 16160825, DOI:
- Engin S. et al. 2010. Benzylguanine Thiol self-assembled monolayers for the immobilization of SNAP-tag proteins on microcontact-printed surface structures Langmuir . ASAP, PubMedID: 20369837, DOI:
- Iversen L. et al. 2008. Templated protein assembly on micro-contact-printed surface patterns. Use of the SNAP-tag protein functionality Langumuir. May 17, PubMedID: 18484753, DOI:
Publications related to SNAP-Capture
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.