SNAP-Capture products are non-magnetic or magnetic agarose beads coupled to a benzylguanine substrate, used to selectively capture and immobilize SNAP-tag® fusion proteins from solution. These beads have a high loading capacity for SNAP-tag fusion proteins and show very low non-specific absorption of proteins from a complex lysate, making them suitable for pull-down applications.
SNAP-tag® is a registered trademark of New England Biolabs, Inc.
- Cellular Imaging & Analysis Brochure
- Iversen L. et al. (2008) Templated protein assembly on micro-contact-printed surface patterns. Use of the SNAP-tag protein functionality Langmuir; May 17, PubMedID: 18484753
- Tugulu S. et al. (2005) Protein-functionalized polymer brushes Biomacromolecules; 6, 1602-1607. PubMedID: 15877383
- Kufer S.K. et al. (2005) Covalent immobilization of recombinant fusion proteins with hAGT for single molecule force spectroscopy Eur Biophys J; 35, 72-78. PubMedID: 16160825
- Engin S. et al. (2010) Benzylguanine Thiol self-assembled monolayers for the immobilization of SNAP-tag proteins on microcontact-printed surface structures Langmuir; ASAP, PubMedID: 20369837
- Maffei, M., Morelli, C., Graham, E., Patriarca, S., Donzelli, L., Doleschall, B., de Castro, Reis, F., Nocchi, L., Chadick, C.H., Reymond, L., Correa, I.R., Jr., Johnsson, K., Hackett, J.A., Heppenstall, P.A (2019) A ligand based system for receptor specific delivery of proteins Sci Rep; 9(1), 19214.. PubMedID: 31844114, DOI: 10.1038/s41598-019-55797-1
- Huber W. et al. (2004) SPR-based interaction studies with small molecular weight ligands using hAGT fusion proteins Anal Biochem; 333, 280-288 . PubMedID: 15450803
- Sielaff I. et al. (2006) Protein function microarrays based on self-immobilizing and self-labeling fusion proteins Chembiochem; 7, 194-202. PubMedID: 16342318
- Jongsma M.A., Litjens R. H. (2006) Self-assembling protein arrays on DNA chips by auto-labeling fusion proteins with a single DNA address Proteomics; 6, 2650-2655 . PubMedID: 16596705
- Kindermann M. et al. (2003) Covalent and selective immobilization of fusion proteins J Am Chem Soc; 125, 7810-7811 . PubMedID: 12822993
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