New England Biolabs offers several buffers optimized for use with our reagents. NEB provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. At 1X concentration, these reaction buffers assure optimal activity of the enzymes. Additional products include gel loading buffers, polymerase buffers, next generation sequencing sample preparation buffers, as well as buffers for protein expression and purification, cloning and RNA manipulation needs.