We have used the Nucleoside Digestion Mix for every single analysis of analog incorporation into RNA. The reagents and protocol provide easy, quick and highly reproducible results to analyze nucleoside mixtures that begin with total RNA from cells.
This digestion mix has transformed our mass spec experiments.
The Nucleoside Digestion Mix significantly simplified our sample preparation protocol and provided us with reproducible results across varying sources of DNA.
I am very happy with the NEB Nucleoside Digestion Mix. Not only does it provide greater levels of digestion, but it allows me to degrade both DNA and RNA simultaneously.
We have used the digestion mix with a wide variety of different nucleotide analogs in both DNA and RNA and found it to work great. It has allowed us to develop new and powerful analytical tools that we now use as part of our continuing effort to optimize our semi-synthetic organisms with expanded genetic alphabets.
This digestion mix was instrumental in getting reliable high quality mass spec data for our DNA modifications.
The Nucleoside Digestion Mix has facilitated sensitive mass spec detection of epigenetic marks in the nucleic acids of various invertebrates we study.
Nucleoside Digestion Mix