A lot of our customers would like to know how they can avoid adapter dimer.
First, make sure that the amount of adaptor you are using is appropriate for your DNA and RNA input, and you can find that information in the kit manual. Then, make sure that you don’t add the adaptor to the ligation master mix. Instead, you can add the adaptor last after you have added everything else to your tube, or you can premix the adaptor with the DNA sample.
Remember, you can always get additional technical support by contacting info@neb.com.
Help us celebrate our 50th anniversary! We have hidden 1,000 golden butterflies and are waiting for you to find them. They can be anywhere that you find NEB! Beginning April 15th, be sure to visit our website and tables at tradeshows and events you are attending. Visit our social media channels frequently for tips on where we have hidden the butterflies – and once you find one, either click or scan the code to be eligible for a 50th anniversary prize pack, as well as a grand prize trip to NEB headquarters in Ipswich, MA!.
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