FAQ: Can reducing agents be used when lysing with NEBExpress® T4 Lysozyme?

Yes, NEBExpress® T4 Lysozyme is compatible with commonly used reducing reagents, including β-mercaptoethanol, DTT, TCEP, and THP. For downstream purification, unlike other vendors, NEBExpress® Nickel Spin Columns (NEB #S1427) and NEBExpress® Nickel Resin (NEB #S1428) are chemically resistant to these reducing reagents.