FAQ: Can Mismatch Endonuclease I function in the presence of other divalent cations?

Other divalent cations, such as Mn2+, Co2+, Ni2+ and Zn2+ will displace the Mg2+ required for activity and inhibit the activity of Mismatch Endonuclease I. The activity of Mismatch Endonuclease I is unaffected by the presence of Ca2+ ions.