HomeFAQsOther companies recommend a 2-min incubation at 25°C for carryover prevention, is a 30-sec incubation at 25°C sufficient?
FAQ: Other companies recommend a 2-min incubation at 25°C for carryover prevention, is a 30-sec incubation at 25°C sufficient?
Yes, given the robust activity of NEB’s thermolabile UDG, we see the degradation of up to approximately 100,000 copies of mock contamination input using a 30-sec incubation step at 25°C. Since carryover contamination typically involves much lower input amounts, there is no need to extend the incubation.
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