HomeFAQsDo NEB Stable Competent E.coli require an outgrowth recovery period after transformation with AmpR plasmids?
FAQ: Do NEB Stable Competent E.coli require an outgrowth recovery period after transformation with AmpR plasmids?
Transformation reactions with AmpR plasmids do not require an outgrowth period after addition of Outgrowth Medium. Plasmid selection using antibiotics other than ampicillin requires an outgrowth period of 60 minutes at 30°C before plating on selective media. 30°C or 37°C may be used for plate incubation, however 30°C is recommended as some constructs may be unstable at elevated temperatures.
Choose your country
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.