FAQ: What is MBP6? Is it different from wild-type MBP produced from E. coli?

MBP6 is the protein produced from pMAL-c6T that utilizes the naturally occurring stop codon following the SbfI recognition sequence. It differs from wild-type MBP by the addition of a methionine at the N-terminus (as do all fusions made in pMAL-c vectors), the mutations that increase the affinity of MBP for the amylose resin, the deletion of the last four residues of wild-type MBP, and the addition of the residues encoded by the spacer and the TEV protease site. MBP6 is a monomer with a molecular weight of 45.5 kDa and a calculated pI of 4.9.