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HomeFAQsCan MBP fusions be purified in the presence of denaturants like urea or guanidine-HCl?
FAQ: Can MBP fusions be purified in the presence of denaturants like urea or guanidine-HCl?
No, MBP’s affinity for amylose and maltose depends on hydrogen bonds that in turn are positioned by the three-dimensional structure of the protein. Agents that interfere with hydrogen bonds or the structure of the protein interfere with binding as well.