HomeFAQsCan the reaction be scaled to smaller or larger volumes?
FAQ: Can the reaction be scaled to smaller or larger volumes?
Yes, the typical reaction volume is 50 µl, but can be scaled up or down linearly. All reaction components, including the DNA template, must be scaled proportionally. The system has been tested with reaction volumes as small as 1 µl and as large as 2 ml. Protein synthesis yields are comparable within this range. For small volumes, such as 1 µl, an acoustic droplet ejection liquid handler (or similar) should be used. For large volumes use a vessel with adequate headspace to allow for aeration and incubate with agitation.
Choose your country
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.