Home FAQs Does Salt-T4 DNA Ligase have a higher tolerance to salt than other ligases?

FAQ: Does Salt-T4 DNA Ligase have a higher tolerance to salt than other ligases?

Yes. While T3 DNA Ligase can tolerate salt concentrations up to 250-300 mM in the reaction, Salt-T4 DNA Ligase can tolerate salt concentrations up to 500 mM in the reaction for cohesive end ligation and up to 150 mM salt concentrations for blunt-end ligation. Please note in the presence of PEG (e.g., StickTogether™ DNA Ligase Buffer), DNA in a high salt solution will precipitate, inhibiting ligation. If you want to ligate DNA in the presence of >100 mM salt, we recommend 1X T4 DNA Ligase Buffer.