FAQ: What types of DNA are suitable for fragmentation using ds DNA Fragmentase (NEB #M0348)?

dsDNA Fragmentase works on dsDNA that is free of enzymatic inhibitors and EDTA. If a DNA sample contains EDTA, the provided MgCl2 can be added to compensate. For consistent fragmentation the amount of EDTA and MgCl2 from sample to sample should be consistent.