HomeFAQsShould the crude extract be diluted with lysis buffer to increase the volume for the binding step?
FAQ: Should the crude extract be diluted with lysis buffer to increase the volume for the binding step?
Dilution of the crude extract may be performed if you are unable to ensure efficient mixing of the beads with the low volume of extract. Dilution of crude extract may result in a slight decrease in yield. In addition, the binding time may be extended up to 1 hour to maximize yield. Another option is to use a sample mixer that provides a vibration function (e.g., HulaMixer, Thermo Catalog #15920D) which is able to agitate the beads and keep them thoroughly re-suspended in small volumes.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.