FAQ: Will Thermolabile Exonuclease I work in other buffers?

Yes, incubation for 4 minutes at 37°C in the following buffers resulted in removal of > 95% of oligos (20 pmol of 20mer ssDNA):

Buffers % of removed oligos
Standard Taq Reaction Buffer  > 95 %
LongAmp® Taq Reaction Buffer > 95 %
OneTaq® Standard Reaction Buffer > 95 %
Isothermal Amplification Buffer > 95 %
Thermopol Reaction Buffer  > 95 %
Epimark® Hot Start Taq Reaction Buffer > 95 %
Q5® Reaction Buffer  > 95 %
AmpliTaq® 360 (Gold) Buffer > 95 %
Phusion® HF Buffer  > 95 %
Green GoTaq® Reaction Buffer > 95 %
NEBuffer™ 1.1  > 95 %
NEBuffer 2.1              > 95 %
NEBuffer 3.1              > 95 %
CutSmart® Buffer  > 95 %