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HomeFAQsIs antibiotic selection necessary to maintain the MiniF-lysY-lacIq plasmid?
FAQ: Is antibiotic selection necessary to maintain the MiniF-lysY-lacIq plasmid?
Although the MiniF plasmid carries the chloramphenicol resistance gene, addition of chloramphenicol is not necessary. If added, chloramphenicol should be at a final concentration of 10 μg/ml. Chloramphenicol levels greater than 15 μg/ml will impair growth.