HomeFAQsAt what temperature is Hi-T7 RNA Polymerase active?
FAQ: At what temperature is Hi-T7 RNA Polymerase active?
Hi-T7 RNA Polymerase is active between 37°C and 56°C. When
performing transcription reactions at temperatures higher than the
suggested 50°C, it is recommended to limit the reaction time to 30
minutes or less as incubation of RNAs at high temperature for
prolonged periods in the presence of MgCl2 (a component of
the Hi-T7 RNA Polymerase Reaction Buffer) may lead to RNA