The first strand cDNA synthesis kits contain different reverse transcriptases with the major differences being their primary applications, format, and reaction temperature. Here is a summary of our three first strand cDNA synthesis kits.
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Product
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LunaScript RT SuperMix Kit (E3010)
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ProtoScript II First Strand cDNA Synthesis Kit (E6560)
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ProtoScript First Strand cDNA Synthesis Kit (E6300)
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| RT | Luna reverse transcriptase | ProtoScript II reverse transcriptase | M-MuLV reverse transcriptase |
| Format | Supermix | Reaction mix, enzyme mix, primer | Reaction mix, enzyme mix, primer |
| Primary Application | Two-step RT-qPCR | Endpoint RT-PCR up to 10kb | Endpoint RT-PCR up to 5kb |
| Secondary Application | Endpoint RT-PCR up to 3kb | Two-step RT-qPCR | Two-step RT-qPCR |
| Reaction Temperature | Up to 65°C (55°C optimal) | Up to 48°C (42°C optimal) | Up to 45°C (42°C optimal) |
| RNA Input | 1μg to 1pg | 1μg to 1pg | 1μg to 1pg |
| Incubation | 25°C 2 min, 55°C 10 min, 95°C 1 min | 25°C 5 min, 42°C 60 min, 85°C 5 min | 25°C 5 min, 42°C 60 min, 85°C 5 min |
| Additional Features | Blue tracking dye, fast 13 min protocol |
