FAQ: What is the optimal pH for Endo F3 activity?

The optimal pH for the cleavage of fucosylated-biantennary (i.e. porcine fibrinogen) and triantennary glycans (i.e. fetuin) by Endo F3 is pH 4.5 (10X Glycobuffer 4).  However, fucosylated-biantennary glycans can also be cleaved by Endo F3 at pH 6.0 (10X Glycobuffer 3).

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Endo F3