HomeFAQsWhat should I do if my target protein is only partially deglycosylated with Rapid PNGase F (non-reducing format)?
FAQ: What should I do if my target protein is only partially deglycosylated with Rapid PNGase F (non-reducing format)?
The amount of Rapid PNGase F (non-reducing format) Buffer can be increased up to 4 µl in a 10 µl total volume to facilitate glycan removal in complex substrates. For some proteins (for instance, Fetuin or chorionic gonadotropin) reducing agents are strictly required and complete rapid deglycosylation under non-reducing conditions will not be feasible. In such cases, we recommend the use of Rapid PNGase F (NEB# P0710).
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.