HomeFAQsAre longer incubation times detrimental to the Rapid PNGase F (non-reducing format) reaction?
FAQ: Are longer incubation times detrimental to the Rapid PNGase F (non-reducing format) reaction?
No. Identical results (quantitative and qualitative yields of N-glycans released as well as intact analysis of the deglycosylated antibody) were achieved following a 10 minute or 1 hour incubation period.
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