HomeFAQsWhat is the optimal heat shock time for the 384-well plate format NEB 5-alpha Competent E. coli (NEB #C2987R)?
FAQ: What is the optimal heat shock time for the 384-well plate format NEB 5-alpha Competent E. coli (NEB #C2987R)?
Both the temperature and the timing of the heat shock step are important and specific to the transformation volume and vessel. Using the 384-well plate provided, 15 seconds at 42°C is optimal. Expect approximately 40% loss in transformation efficiency when heat shocking for 20 seconds.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.