HomeFAQsCan I use β1–4 Galactosidase S in a double digest with other exoglycosidases and/or endoglycosidases?
FAQ: Can I use β1–4 Galactosidase S in a double digest with other exoglycosidases and/or endoglycosidases?
Yes. We recommend double digesting β1–4 Galactosidase S with other exoglycosidases using 1X GlycoBuffer 1 (50mM NaAcetate, pH 5.5, 5mM CaCl2). We recommend using 1X G7 Reaction Buffer (50 mM sodium phosphate, pH 7.5) for digestion of β1–4 Galactosidase S with PNGase F and/or O-Glycosidase.