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HomeFAQsCan I use α2-3,6,8,9 Neuraminidase A in a double digest with other exoglycosidases and/or endoglycosidases?
FAQ: Can I use α2-3,6,8,9 Neuraminidase A in a double digest with other exoglycosidases and/or endoglycosidases?
Yes. We recommend double digesting α2-3,6,8,9 Neuraminidase A with other exoglycosidases using 1X GlycoBuffer 1 (50mM NaAcetate, pH 5.5, 5mM CaCl2). We recommend using 1X G7 Reaction Buffer (50 mM sodium phosphate, pH 7.5) for digestion of α2-3,6,8,9 Neuraminidase A with PNGase F and/or O-Glycosidase.