StuI is blocked by overlapping dcm methylation if the site ccAGGCCT or AGGCCTgg occurs. To avoid dcm methylation, use a dcm-deficient strain such as dam-/dcm-Competent E.coli (NEB Catalog #C2925 ). For up-to-date information about methylation sensitivities, please visit Dam-Dcm and CpG Methylation or REBASE.
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